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KMID : 0357119930150020201
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Korean Journal of Immunology
1993 Volume.15 No. 2 p.201 ~ p.207
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Effect of Calcium ion on fMLP-stimulated Neutrophils Chemotaxis
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Hwang Jeong-Won
Jung Noh-Pal
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Abstract
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Chemotaxis of neutrophils stimulated by fMLP(N-formyl-methionylleucylphenyl-alanine) is not fully understood process. Present study mainly deals with the role of Ca" as a second messenger in chemotaxis. The chemotaxis of neutrophils which were treated with calcium antagonist and calcium channel blocker was decreased significantly. To investigate the effect of extracellular and intraceI-lular calcium, EGTA or EGTA +A23187 were treated. Both treatments decreased the chemotaxis of neutrophils. Intracellular Ca´ level change was checked using fluorescent dye Quin-2/Am. When treated with fMLP, intractellular Cap+ level was found to be dependent on both extracellular Cal+ uptake and release from internal Cat+ storages. On the other hand, the treatment of callmodulin antagonists did not have any significant effect on chemotaxis. When treated with varying concentration of PK-C inhibitor, chemotaxis was decreased dependent manner on concentration. In addition to Ca´´, PK-C is also supposed to be related to the signal transdution process of chemotaxis induced by fMLP.
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KEYWORD
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Ca2+, fMLP, Neutrophils, Chemotaxis
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